5D75
Crystal structure of Human FKBD25 in complex with FK506
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.784, 74.784, 44.618 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.830 |
| R-factor | 0.19073 |
| Rwork | 0.187 |
| R-free | 0.23311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.549 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.045 | 0.556 |
| Number of reflections | 12952 | |
| <I/σ(I)> | 34.4 | 2 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 5.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 30% v/v Jeffamine ED-2001, 0.1M HEPES pH 7.0 |
