5D6F
Structure of human methionine aminopeptidase-2 complexed with spiroepoxytriazole inhibitor (+)-31b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97895 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.110, 99.500, 101.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.14874 |
| Rwork | 0.147 |
| R-free | 0.18502 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cls |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.073 | 0.750 |
| Number of reflections | 65507 | |
| <I/σ(I)> | 11.44 | 2.04 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 4.37 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | Na Citrate, Tert-butanol |
