5D5H
Crystal structure of Mycobacterium tuberculosis Topoisomerase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.002, 93.149, 140.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.781 - 2.520 |
R-factor | 0.1769 |
Rwork | 0.174 |
R-free | 0.24090 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.560 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.098 | 0.696 |
Number of reflections | 28858 | |
<I/σ(I)> | 14 | 2 |
Completeness [%] | 99.2 | 97.6 |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M ammonium Sulfate, 0.1M Tris:HCl, 25% (w/v) PEG3350 |