5D5A
In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0322 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.000, 170.000, 40.500 |
Unit cell angles | 90.00, 106.25, 90.00 |
Refinement procedure
Resolution | 43.960 - 2.483 |
R-factor | 0.2142 |
Rwork | 0.212 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rh1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.487 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.483 | 2.483 |
Number of reflections | 23086 | |
<I/σ(I)> | 7.27 | 1.11 |
Completeness [%] | 95.1 | 91 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol |