5D5A

In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-2 adrenergic receptor,Endolysin,Beta-2 adrenergic receptor	polymer	500	56601.4	1	UniProt (P07550) UniProt (P00720) Pfam (PF00001) Pfam (PF00959)	Homo sapiens (Human)	Beta-2 adrenoreceptor,Beta-2 adrenoceptor,Lysis protein,Lysozyme,Muramidase,Beta-2 adrenoreceptor,Beta-2 adrenoceptor
2	B (B)	alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900018) GlyTouCan (G66120GK)		beta-maltose
3	C (A)	(2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol	non-polymer		298.4	1	Chemie (CAU)
4	D, E (A)	1,4-BUTANEDIOL	non-polymer		90.1	2	Chemie (BU1)
5	F (A)	ACETAMIDE	non-polymer		59.1	1	Chemie (ACM)
6	G, H, I (A)	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)
7	J (A)	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)
8	K (A)	DODECAETHYLENE GLYCOL	non-polymer		546.6	1	Chemie (12P)
9	L, M, N, O, P... (A)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
10	R (A)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

A: 1 - 230 (UniProt: P07550)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Arg 16	Gly 16	conflict
Gln 27	Glu 27	conflict
Glu 187	Asn 187	engineered mutation

A: 1002 - 1161 (UniProt: P00720)

PDB	External Database	Details
Gly 1012	Arg 12	conflict
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Arg 1137	Ile 137	conflict

A: 263 - 365 (UniProt: P07550)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56601.4
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	15
	Total formula weight	3077.1
All*	Total formula weight	60020.8

*Water molecules are not included.