5D10

Kinase domain of cSrc in complex with RL236

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-07-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5417
Spacegroup name	P 1
Unit cell lengths	41.900, 63.500, 76.600
Unit cell angles	79.20, 88.10, 90.10

Refinement procedure

Resolution	44.110 - 2.700
R-factor	0.2724
Rwork	0.269
R-free	0.35920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oiq
RMSD bond length	0.014
RMSD bond angle	1.892
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	2.800
High resolution limit [Å]	2.700	16.000	2.700
Rmerge	0.066	0.029	0.183
Rmeas	0.086	0.038	0.238
Total number of observations	44802
Number of reflections	18728	86	2102
<I/σ(I)>	12.17	23.82	5.73
Completeness [%]	87.9	82.7	94
Redundancy	2.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.085-0.125 mM MES, 9-11.5 % PEG20000