5CX6
Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with CDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2011-04-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 133.700, 149.600, 100.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.850 - 2.100 |
R-factor | 0.1861 |
Rwork | 0.184 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xhi |
RMSD bond length | 0.007 |
RMSD bond angle | 1.062 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.850 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.115 | 0.422 |
Number of reflections | 115494 | |
<I/σ(I)> | 5.64 | 1.8 |
Completeness [%] | 98.3 | 98.6 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 8000, Lithium sulfate |