5CU5
Crystal structure of ERAP2 without catalytic Zn(II) atom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.650, 135.170, 126.490 |
| Unit cell angles | 90.00, 90.74, 90.00 |
Refinement procedure
| Resolution | 48.561 - 3.020 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e36 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.610 | 3.180 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.139 | 0.969 |
| Number of reflections | 49350 | |
| <I/σ(I)> | 10.3 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 6 mg/ml protein, 7 %(w/v) PEG 8000, 20 %(v/v) ethylene glycol, 69 mM MES, 31 mM imidazole |
