5CP6
Nucleosome Core Particle with Adducts from the Anticancer Compound, [(eta6-5,8,9,10-tetrahydroanthracene)Ru(ethylenediamine)Cl][PF6]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2013-09-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.50 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.570, 109.570, 182.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.390 - 2.600 |
R-factor | 0.21102 |
Rwork | 0.210 |
R-free | 0.25064 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wu8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.446 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 76.390 |
High resolution limit [Å] | 2.600 |
Number of reflections | 57728 |
<I/σ(I)> | 15.1 |
Completeness [%] | 86.3 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40 mM MnCl2, 30 mM KCl, 20 mM K-Cacodylate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |