5CM9
Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97488 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.877, 99.828, 111.487 |
Unit cell angles | 90.00, 127.00, 90.00 |
Refinement procedure
Resolution | 46.630 - 2.600 |
R-factor | 0.27573 |
Rwork | 0.273 |
R-free | 0.31988 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4JGW as poly Ala model |
RMSD bond length | 0.006 |
RMSD bond angle | 0.925 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.127 | |
Number of reflections | 40751 | |
<I/σ(I)> | 12.72 | 2.69 |
Completeness [%] | 88.5 | 56.8 |
Redundancy | 2.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.45 | 289 | 15% PEG 3350, 200 mM ammonium acetate, 100 mM Bis-Tris propane |