5CL1
Complex structure of Norrin with human Frizzled 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 143.291, 150.589, 92.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.800 |
R-factor | 0.2639 |
Rwork | 0.262 |
R-free | 0.31050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4my2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.770 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.910 |
High resolution limit [Å] | 3.800 | 8.690 | 3.800 |
Rmerge | 0.264 | 0.090 | 0.923 |
Rmeas | 0.280 | 0.097 | |
Rpim | 0.117 | 0.035 | 0.526 |
Total number of observations | 107966 | ||
Number of reflections | 18303 | ||
<I/σ(I)> | 3.5 | ||
Completeness [%] | 90.5 | 99.9 | 65.2 |
Redundancy | 5.9 | 7.4 | 3.3 |
CC(1/2) | 0.997 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 200 mM ammonium sulfate, 100 mM sodium cacodylate trihydrate (pH 6.5), 15% w/v polyethylene glycol 8,000 |