5CKS
DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase in complex with DAHP Oxime.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-04-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 209.990, 53.360, 150.655 |
Unit cell angles | 90.00, 115.44, 90.00 |
Refinement procedure
Resolution | 42.424 - 2.118 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kfl |
RMSD bond length | 0.007 |
RMSD bond angle | 1.025 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.160 |
High resolution limit [Å] | 2.118 | 2.118 |
Rmerge | 0.093 | 0.406 |
Number of reflections | 85303 | |
<I/σ(I)> | 12.8 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Mother liquor (20% PEG 3350, 200 mM trilithium citrate tetrahydrate, 6% galactose) was mixed in equal volume with DHAPS at 10 mg/ml in buffer containing 20 mM Tris-HCl, 0.1 mM TCEP. |