5CJ7
Human DNA polymerase lambda L431A mutant- MgdTTP binary and complex with 6 paired DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 84.029, 84.029, 398.122 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.505 - 2.901 |
R-factor | 0.2135 |
Rwork | 0.209 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xsl |
RMSD bond length | 0.007 |
RMSD bond angle | 0.958 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
Rmerge | 0.165 | 0.047 | 0.860 |
Total number of observations | 281822 | ||
Number of reflections | 19619 | ||
<I/σ(I)> | 7.2 | ||
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 14.4 | 16.1 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate |