5CIL
Crystal Structure of non-neutralizing version of 4E10 (WDWD) with epitope bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.300, 44.890, 86.160 |
| Unit cell angles | 90.00, 114.53, 90.00 |
Refinement procedure
| Resolution | 44.500 - 1.810 |
| R-factor | 0.16408 |
| Rwork | 0.163 |
| R-free | 0.18838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wy7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.099 | 0.667 |
| Number of reflections | 50189 | |
| <I/σ(I)> | 11.9 | 2.8 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 100mM TRIS-HCl, 200mM Ammonium acetate, 34%PEG 4,000 |
