5CEY
Crystal Structure of Fab 9H+3L, a putative precursor of the PGT121 family of potent HIV-1 antibodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.736, 338.146, 62.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.747 - 2.387 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r26 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.747 | |
| High resolution limit [Å] | 2.387 | 2.400 |
| Number of reflections | 33105 | |
| <I/σ(I)> | 7.87 | 4.7 |
| Completeness [%] | 94.2 | 72.2 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.14 | 40% (v/v) PEG 600, 0.1M NaCl and 0.1 M Na citrate pH 5.5 |
