5CAY

Envelope glycoprotein gp120 core from HIV type 2 bound to the first two domains of human soluble CD4 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	94.276, 100.223, 199.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.570 - 3.000
R-factor	0.23545
Rwork	0.231
R-free	0.28673
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nxy 3lqa
RMSD bond length	0.008
RMSD bond angle	1.486
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.570
High resolution limit [Å]	3.000
Rmerge	0.081
Rpim	0.042
Number of reflections	19116
<I/σ(I)>	12.8
Completeness [%]	99.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.15M DL-Malic acid 7.0, 0.1M imidazole pH 7.0, 22% PEGMME 550