5C8W
PKG II's Amino Terminal Cyclic Nucleotide Binding Domain (CNB-A) in a complex with cGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2013-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .9774 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.710, 103.454, 176.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.727 - 1.800 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.19550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c8w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.480 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.727 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.147 | 0.470 |
| Number of reflections | 79944 | |
| <I/σ(I)> | 14 | 3.3 |
| Completeness [%] | 99.6 | 94.4 |
| Redundancy | 12.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 33 mM cGMP, 200 mM sodium malonate, 5-10% ethylene glycol, 20% PEG |
