5C6W
anti-CXCL13 scFv - E10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2012-11-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.980, 80.360, 119.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.540 |
R-factor | 0.1544 |
Rwork | 0.154 |
R-free | 0.16350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3juy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.044 | 0.496 |
Number of reflections | 98395 | |
<I/σ(I)> | 26 | 3.4 |
Completeness [%] | 98.8 | 92.2 |
Redundancy | 8.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM Tris pH 8.5, 2000 mM (NH4)2SO4 |