5C6H
Mcl-1 complexed with Mule
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2014-05-18 |
Detector | APEX II CCD |
Wavelength(s) | 0.987, 0.988, 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 31.790, 114.240, 135.980 |
Unit cell angles | 90.12, 92.32, 90.01 |
Refinement procedure
Resolution | 35.451 - 2.050 |
R-factor | 0.2974 |
Rwork | 0.295 |
R-free | 0.34560 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.654 |
Data reduction software | XDS (IA32) |
Phasing software | Coot (0.8.1.1) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.451 | 2.750 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 114746 | |
<I/σ(I)> | 3.26 | |
Completeness [%] | 95.6 | |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG3350, calcium chloride |