5C6D
Crystal structure of USP7 in complex with UHRF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9774 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.606, 62.430, 98.505 |
Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
Resolution | 49.251 - 2.292 |
R-factor | 0.1884 |
Rwork | 0.186 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ylm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.043 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.065 | 0.525 |
Number of reflections | 36467 | |
<I/σ(I)> | 16.64 | 2.2 |
Completeness [%] | 98.3 | 99.4 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM CHES (pH 9.0), 20% PEG8000 |