5C6D
Crystal structure of USP7 in complex with UHRF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.606, 62.430, 98.505 |
| Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
| Resolution | 49.251 - 2.292 |
| R-factor | 0.1884 |
| Rwork | 0.186 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ylm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.043 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.065 | 0.525 |
| Number of reflections | 36467 | |
| <I/σ(I)> | 16.64 | 2.2 |
| Completeness [%] | 98.3 | 99.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM CHES (pH 9.0), 20% PEG8000 |
