5C51

Probing the Structural and Molecular Basis of Nucleotide Selectivity by Human Mitochondrial DNA Polymerase gamma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	215.049, 215.049, 161.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.017 - 3.426
R-factor	0.3161
Rwork	0.315
R-free	0.34490
RMSD bond length	0.003
RMSD bond angle	0.755
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.420
Number of reflections	50770
<I/σ(I)>	2
Completeness [%]	95.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	PEG4000, KCl, HEPES