5C51
Probing the Structural and Molecular Basis of Nucleotide Selectivity by Human Mitochondrial DNA Polymerase gamma
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 215.049, 215.049, 161.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.017 - 3.426 |
R-factor | 0.3161 |
Rwork | 0.315 |
R-free | 0.34490 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.755 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.420 |
Number of reflections | 50770 |
<I/σ(I)> | 2 |
Completeness [%] | 95.0 |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | PEG4000, KCl, HEPES |