5C46

Crystal structure of an engineered construct of phosphatidylinositol 4 kinase III beta in complex with GTP gamma S loaded Rab11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	48.940, 97.950, 190.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.975 - 2.650
R-factor	0.2177
Rwork	0.216
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d0l
RMSD bond length	0.003
RMSD bond angle	0.551
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.980	48.980	2.780
High resolution limit [Å]	2.650	8.790	2.650
Rmerge	0.110	0.043	0.920
Rpim	0.047	0.020	0.405
Total number of observations	173010	4567	22088
Number of reflections	27529
<I/σ(I)>	12.2	41.6	2.6
Completeness [%]	100.0	98.9	99.9
Redundancy	6.3	5.3	6.2
CC(1/2)	0.997	0.998	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	290	PEG-4000, sodium citrate, ammonium sulfate, glycerol