5C0Y
Crystal structure of the Rrp6 catalytic domain bound to poly(U) RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-20 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 31 |
| Unit cell lengths | 110.186, 110.186, 78.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.710 - 2.100 |
| R-factor | 0.1507 |
| Rwork | 0.148 |
| R-free | 0.18010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.710 | 47.710 | 2.150 |
| High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
| Rmerge | 0.051 | 0.015 | |
| Rpim | 0.022 | 0.006 | 0.553 |
| Total number of observations | 402597 | 4713 | 27535 |
| Number of reflections | 62575 | ||
| <I/σ(I)> | 22.5 | 103.2 | 1.5 |
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 6.4 | 6.7 | 5.9 |
| CC(1/2) | 1.000 | 1.000 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 25% PEG3350, 100 mM Tris pH 8.5 |
