5C0Y

Crystal structure of the Rrp6 catalytic domain bound to poly(U) RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99999
Spacegroup name	P 31
Unit cell lengths	110.186, 110.186, 78.883
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.710 - 2.100
R-factor	0.1507
Rwork	0.148
R-free	0.18010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.260
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.710	47.710	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.051	0.015
Rpim	0.022	0.006	0.553
Total number of observations	402597	4713	27535
Number of reflections	62575
<I/σ(I)>	22.5	103.2	1.5
Completeness [%]	100.0	99.2	100
Redundancy	6.4	6.7	5.9
CC(1/2)	1.000	1.000	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	25% PEG3350, 100 mM Tris pH 8.5