5C0W

Structure of a 12-subunit nuclear exosome complex bound to single-stranded RNA substrates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2398
Spacegroup name	P 32 2 1
Unit cell lengths	195.380, 195.380, 463.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.090 - 4.600
R-factor	0.2773
Rwork	0.271
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ifd 4wfc 4wfd 4oo1 2vnu 2hbj 4wp8
RMSD bond length	0.013
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.090		4.800
High resolution limit [Å]	4.600	20.000	4.600
Rmerge	0.114	0.082	0.708
Rmeas	0.126	0.091	1.002
Total number of observations	138355
Number of reflections	40273	770	1662
<I/σ(I)>	6	16.98	0.45
Completeness [%]	69.5	92.1	24.3
Redundancy	3.435		1.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	4.1-4.4%(w/v) PEG 8000, 0.1M MES pH 5.5, 0.2M KCl, 0.01M MgCl2, 283K and 292K