5C09
HLA class I histocompatibility antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.830, 100.070, 122.440 |
| Unit cell angles | 96.91, 98.31, 96.52 |
Refinement procedure
| Resolution | 98.457 - 2.475 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3UTP and 3UTQ |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.743 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.457 | 98.457 | 2.540 |
| High resolution limit [Å] | 2.475 | 11.070 | 2.470 |
| Rmerge | 0.074 | 0.563 | |
| Rmeas | 0.127 | ||
| Rpim | 0.084 | 0.043 | 0.407 |
| Total number of observations | 140157 | 1633 | 10379 |
| Number of reflections | 70968 | ||
| <I/σ(I)> | 4.9 | 7.8 | 2.1 |
| Completeness [%] | 98.2 | 96.8 | 97.5 |
| Redundancy | 2 | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M sodium cacodylate pH6.5, 15% PEG 4000, 0.2M sodium acetate |
