5C01
Crystal Structure of kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-08-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.340, 48.170, 114.210 |
Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.150 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.24030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.338 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.373 | 49.336 | 2.270 |
High resolution limit [Å] | 2.150 | 6.800 | 2.150 |
Rmerge | 0.043 | 0.411 | |
Rmeas | 0.116 | ||
Rpim | 0.060 | 0.026 | 0.264 |
Total number of observations | 119960 | 4077 | 15631 |
Number of reflections | 33558 | ||
<I/σ(I)> | 8.9 | 18.5 | 2.8 |
Completeness [%] | 99.6 | 99.7 | 98.8 |
Redundancy | 3.6 | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 18-22% PEG 4000, 0.1 M Tris, pH 8.5, 200 mM CaCl2 |