5BZZ

Crystal structure of human phosphatase PTEN in its reduced state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.00001
Spacegroup name	C 2 2 21
Unit cell lengths	207.170, 207.390, 87.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.360 - 2.200
R-factor	0.1934
Rwork	0.192
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d5r
RMSD bond length	0.009
RMSD bond angle	1.567
Data scaling software	XSCALE
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.364		2.300
High resolution limit [Å]	2.200	20.000	2.200
Rmerge	0.150	0.048	2.637
Rmeas	0.156	0.052	2.739
Total number of observations	1293017
Number of reflections	95899	152	11832
<I/σ(I)>	13.96	49.07	1.17
Completeness [%]	99.9	90.5	99.9
Redundancy	13.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES pH 6.5, 1.25 M L-tartrate, 7.5% glycerol