5BV8
G1324S mutation in von Willebrand Factor A1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 61 |
| Unit cell lengths | 86.450, 86.450, 68.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.230 - 1.590 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c29 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.021 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.230 |
| High resolution limit [Å] | 1.590 |
| Rmerge | 0.081 |
| Number of reflections | 38781 |
| <I/σ(I)> | 14 |
| Completeness [%] | 100.0 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | 30% PEG 400, 100 mM CAPS/Sodium hydroxide |
