5BV6

PKG II's Carboxyl Terminal Cyclic Nucleotide Binding Domain (CNB-B) in a complex with cGMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.344, 50.479, 69.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.770 - 1.940
R-factor	0.179
Rwork	0.177
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ku7
RMSD bond length	0.009
RMSD bond angle	1.177
Data reduction software	iMOSFLM
Phasing software	HKL-3000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.775
High resolution limit [Å]	1.940
Number of reflections	10872
<I/σ(I)>	37.7
Completeness [%]	99.5
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	20 mM CaCl2, 100 mM NaOAc, 30% MPD