5BTG
Crystal structure of a topoisomerase II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.094, 82.989, 129.101 |
| Unit cell angles | 90.00, 108.76, 90.00 |
Refinement procedure
| Resolution | 46.325 - 2.500 |
| R-factor | 0.2268 |
| Rwork | 0.226 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bs8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.689 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.330 | 2.589 | |
| High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
| Rmerge | 0.139 | 0.026 | |
| Rmeas | 0.162 | 0.036 | 1.364 |
| Total number of observations | 283517 | ||
| Number of reflections | 283502 | 1611 | 10601 |
| <I/σ(I)> | 8.93 | 22.06 | 1.37 |
| Completeness [%] | 99.7 | 96.6 | 99.57 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400 |
