5BTD
Crystal structure of a topoisomerase II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.907, 83.158, 129.203 |
| Unit cell angles | 90.00, 108.65, 90.00 |
Refinement procedure
| Resolution | 47.394 - 2.497 |
| R-factor | 0.2162 |
| Rwork | 0.215 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bs8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.741 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.394 | 2.586 | |
| High resolution limit [Å] | 2.497 | 11.170 | 2.497 |
| Rmerge | 0.137 | 0.019 | |
| Rmeas | 0.158 | 0.027 | 1.727 |
| Total number of observations | 285078 | ||
| Number of reflections | 285133 | 1567 | 10299 |
| <I/σ(I)> | 10.36 | 32.28 | 1.1 |
| Completeness [%] | 99.7 | 93.4 | 97.56 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400 |
