5BT2
MeCP2 MBD domain (A140V) in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.660, 53.490, 65.780 |
| Unit cell angles | 90.00, 132.47, 90.00 |
Refinement procedure
| Resolution | 48.500 - 2.200 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c2i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.830 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.500 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 10482 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 98.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG 2000, 200 mM ammonium acetate, 10 mM magnesium acetate, 50 mM sodium cacodylate |
