5B4X

Crystal structure of the ApoER2 ectodomain in complex with the Reelin R56 fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97904
Spacegroup name	P 65
Unit cell lengths	205.951, 205.951, 169.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.520 - 3.200
R-factor	0.20277
Rwork	0.200
R-free	0.25481
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.178
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.139
Number of reflections	67441
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	14.8
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	11-12.5%(wt./vol.) PEG 3350 200-250mM sodium thiocyanate, 20mM HEPES-Na (pH 7.5)