5AXN

Crystal structure of Thg1 like protein (TLP) with tRNA(Phe) and GDPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	82.255, 134.052, 147.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.762 - 2.703
R-factor	0.2274
Rwork	0.225
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wbz 1ehz
RMSD bond length	0.007
RMSD bond angle	0.803
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.700	2.700
Rmerge		0.817
Number of reflections	22669
<I/σ(I)>	16.9	2.5
Completeness [%]	99.7	99.3
Redundancy	8.1	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 3350, tri-potassium citrate