3WBZ

Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with ATP

Summary for 3WBZ

• Entry DOI: 10.2210/pdb3wbz/pdb
• Related: 3WC0 3WC1 3WC2
• Descriptor: Likely histidyl tRNA-specific guanylyltransferase, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: transferase
• Biological source: Candida albicans (Yeast)
• Total number of polymer chains: 8
• Total formula weight: 270212.20

Authors

Nakamura, A.,Nemoto, T.,Sonoda, T.,Yamashita, K.,Tanaka, I.,Yao, M. (deposition date: 2013-05-24, release date: 2013-12-18, Last modification date: 2023-11-08)

Primary citation

Nakamura, A.,Nemoto, T.,Heinemann, I.U.,Yamashita, K.,Sonoda, T.,Komoda, K.,Tanaka, I.,Soll, D.,Yao, M.
Structural basis of reverse nucleotide polymerization
Proc.Natl.Acad.Sci.USA, 110:20970-20975, 2013

PubMed Abstract: Nucleotide polymerization proceeds in the forward (5'-3') direction. This tenet of the central dogma of molecular biology is found in diverse processes including transcription, reverse transcription, DNA replication, and even in lagging strand synthesis where reverse polymerization (3'-5') would present a "simpler" solution. Interestingly, reverse (3'-5') nucleotide addition is catalyzed by the tRNA maturation enzyme tRNA(His) guanylyltransferase, a structural homolog of canonical forward polymerases. We present a Candida albicans tRNA(His) guanylyltransferase-tRNA(His) complex structure that reveals the structural basis of reverse polymerization. The directionality of nucleotide polymerization is determined by the orientation of approach of the nucleotide substrate. The tRNA substrate enters the enzyme's active site from the opposite direction (180° flip) compared with similar nucleotide substrates of canonical 5'-3' polymerases, and the finger domains are on opposing sides of the core palm domain. Structural, biochemical, and phylogenetic data indicate that reverse polymerization appeared early in evolution and resembles a mirror image of the forward process.

PubMed: 24324136
DOI: 10.1073/pnas.1321312111

Experimental method

X-RAY DIFFRACTION (2.392 Å)