5AWM
The Crystal Structure of JNK from Drosophila melanogaster Reveals an Evolutionarily Conserved Topology with that of Mammalian JNK Proteins.
Replaces: 4M3AExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.486, 55.329, 126.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.280 - 1.790 |
| R-factor | 0.17936 |
| Rwork | 0.175 |
| R-free | 0.21956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xs0 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.982 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.280 | 1.660 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.062 | 0.794 |
| Number of reflections | 51133 | |
| <I/σ(I)> | 0.118 | 1.8 |
| Completeness [%] | 99.4 | 97.6 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 25% (w/v) PEG 4000, 0.1 M Tris-HCl pH 8.5 and 0.2 M MgCl2 |
