5ANM
Crystal structure of IgE Fc in complex with a neutralizing antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.957, 140.957, 244.448 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.290 - 2.850 |
| R-factor | 0.1988 |
| Rwork | 0.196 |
| R-free | 0.25993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1AQK 1fp5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.018 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.110 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 66338 | |
| <I/σ(I)> | 20.2 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM MGCL2, 100 MM SODIUM CITRATE PH 5.0 AND 15% PEG 4000 |
