Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-21 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.670, 57.590, 65.560 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 43.260 - 1.960 |
| R-factor | 0.21553 |
| Rwork | 0.214 |
| R-free | 0.25179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uwy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.260 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 51344 | |
| <I/σ(I)> | 12.3 | 2.6 |
| Completeness [%] | 96.0 | 95.6 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20 % PEG 5K MME, 0.1 M TRIS, PH 7.5, 0.2 M (NH4)2SO4 |
