5AGI
Crystal structure of the LeuRS editing domain of Candida albicans Mutant K510A in complex with the adduct formed by AN2690-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.176, 58.427, 95.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.790 - 1.470 |
| R-factor | 0.19994 |
| Rwork | 0.199 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.192 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.790 | 1.510 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 38517 | |
| <I/σ(I)> | 37.4 | 4.2 |
| Completeness [%] | 99.3 | 91.2 |
| Redundancy | 9.1 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.2 M SODIUM ACETATE, 0.1 M HEPES SODIUM PH 7.4, 30% PEG 2000 |
