5AGA
Crystal structure of the Helicase domain of human DNA polymerase theta in complex with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-02 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 116.170, 132.710, 156.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.632 - 2.900 |
R-factor | 0.2198 |
Rwork | 0.217 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.002 |
RMSD bond angle | 0.533 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.630 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.070 | 1.090 |
Number of reflections | 26924 | |
<I/σ(I)> | 22.8 | 1.9 |
Completeness [%] | 99.3 | 98.5 |
Redundancy | 5.6 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 19% PEG 3350, 0.2M POTASSIUM CITRATE TRIBASIC |