5AFW
Assembly of methylated LSD1 and CHD1 drives AR-dependent transcription and translocation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.296, 44.445, 46.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.190 - 1.600 |
| R-factor | 0.2103 |
| Rwork | 0.209 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b2w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.150 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.030 | 1.410 |
| Number of reflections | 30650 | |
| <I/σ(I)> | 16.4 | 0.8 |
| Completeness [%] | 98.8 | 83.9 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.5, 0.2 M L-PROLINE, 10% (W/V) PEG3350 |
