5AER
Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with D2 dopamine receptor peptide from Homo sapiens
Replaces: 2YOUExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH MARMOSAIC 300MM |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 44.670, 44.670, 205.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.380 - 2.190 |
| R-factor | 0.22051 |
| Rwork | 0.217 |
| R-free | 0.25577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 11235 | |
| <I/σ(I)> | 15 | 2.4 |
| Completeness [%] | 97.1 | 77.8 |
| Redundancy | 5.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
