5AEK
Crystal structure of the human SENP2 C548S in complex with the human SUMO1 K48M F66W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 113.721, 119.319, 199.840 |
Unit cell angles | 90.00, 89.67, 90.00 |
Refinement procedure
Resolution | 47.000 - 3.000 |
R-factor | 0.25935 |
Rwork | 0.257 |
R-free | 0.32625 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tgz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.596 |
Data reduction software | XPS |
Data scaling software | CCP4I |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.760 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.160 | 0.460 |
Number of reflections | 101157 | |
<I/σ(I)> | 3.6 | 1.4 |
Completeness [%] | 94.5 | 87.1 |
Redundancy | 2.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 291 | 2M AMMONIUM SULFATE, 5% PEG 400, 0.1M BIS-TRIS PH 6.5 291K |