5A3I
Crystal Structure of a Complex formed between FLD194 Fab and Transmissible Mutant H5 Haemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.750, 87.700, 100.900 |
| Unit cell angles | 78.67, 84.66, 73.91 |
Refinement procedure
| Resolution | 83.330 - 2.890 |
| R-factor | 0.24547 |
| Rwork | 0.244 |
| R-free | 0.27689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4BH2 3ztn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.845 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.330 | 2.990 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 57328 | |
| <I/σ(I)> | 13.1 | 1.9 |
| Completeness [%] | 91.4 | 78.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 7.5, 10% PEG 4000, 16% ISOPROPANOL |
