5ZNN

Crystal structure of ligand-free form of the Vps10 ectodomain of Sortilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	80.264, 138.496, 146.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.607 - 2.448
R-factor	0.1814
Rwork	0.180
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4po7
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.448	2.450
Number of reflections	60757	2997
<I/σ(I)>	22.6	2.5
Completeness [%]	99.6	99.7
Redundancy	4.6	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	23% (w/v) polyethylene glycol monomethyl ether 550 (Sigma), 0.4 M sodium acetate pH 4.6