5ZMJ
Crystal structure of the Fab region of a neutralizing fully human antibody against GM-CSF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.120, 66.690, 129.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.370 - 1.810 |
| R-factor | 0.16272 |
| Rwork | 0.161 |
| R-free | 0.19133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eow |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.796 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.370 | 1.850 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.068 | 0.171 |
| Number of reflections | 43756 | 3133 |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 98.1 | |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M Potassium sodium tartrate tetrahydrate, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 2.0M Ammonium sulfate |
