5ZM8
Crystal structure of ORP2-ORD in complex with PI(4,5)P2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 156.160, 156.160, 199.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.322 - 2.700 |
R-factor | 0.2178 |
Rwork | 0.216 |
R-free | 0.25430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zm5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.122 | 0.553 |
Rmeas | 0.129 | 0.588 |
Rpim | 0.042 | 0.198 |
Number of reflections | 34082 | 1676 |
<I/σ(I)> | 17.8 | 4.38 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 9.9 | 8.6 |
CC(1/2) | 0.932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100mM MES pH 6.5, 50mM MgCl2, 8% isopropanol, 1.5% PEG3350 |