5ZK7

Stapled-peptides tailored against initiation of translation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.983998
Spacegroup name	P 21 21 21
Unit cell lengths	57.350, 68.170, 111.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.890 - 2.120
R-factor	0.21825
Rwork	0.216
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tpw
RMSD bond length	0.007
RMSD bond angle	1.353
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.120	2.120
Number of reflections	25155	3804
<I/σ(I)>	13.49	2.7
Completeness [%]	99.8
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2M Potassium Iodide, 0.1M MES/Imidazole pH6.5, 25%(wv) PEG4000