5ZJF
LDHA-MA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.226, 141.495, 144.079 |
| Unit cell angles | 90.00, 110.18, 90.00 |
Refinement procedure
| Resolution | 37.100 - 2.602 |
| R-factor | 0.1839 |
| Rwork | 0.181 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4okn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.630 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 153798 | 560845 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 99.4 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 8% Tacsimate (pH 7.0), 20% Polyethylene glycol (PEG) 3350 |
