5ZJE

LDHA-mla

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	85.588, 219.333, 112.788
Unit cell angles	90.00, 92.28, 90.00

Refinement procedure

Resolution	30.190 - 2.929
R-factor	0.1928
Rwork	0.188
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4okn
RMSD bond length	0.010
RMSD bond angle	1.241
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.200	2.962
High resolution limit [Å]	2.929	2.929
Rmerge	0.168	0.385
Number of reflections	86824	1694609
<I/σ(I)>	11.4
Completeness [%]	97.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.2M soduim malonnate, 20% Polyethylene glycol(PEG) 3350